Obed Trinurcahyo Kinantyo Paundralingga, Darmawan Darkim, Badrul Munir, Bonaventura Handoko Daeng
  MNJ, pp. 73-77  


Background. Stress of varying duration and types are known to affect the number and activation level of cerebral mast cells (MCs) via plasma CRH. Although MC number is known not to be increased in acute stress, elevated plasma CRH might still activate brain MCs.
Objective. To investigate the effect of acute stress of incremental duration to the activation level of thalamic and hippocampal mast cells using elevated platform test to elicit stress in male Wistar rats.
Methods. This research used randomized post-test only control group design with 4 control group of 30, 60, and 90 minute stress exposure. Mast cell activation of the regiotalamus and hippocampus is assessed by histomorphometrics.
Results. In the hippocampus, we found a significant difference of MC activation between control and experimental groups (p=0.014; p<0.05) but not among the incremental duration of acute stress. However, MC activation was not different between control and experimental groups in the thalamus.
Conclusion. Acute stress exposure increases MC activation without recruiting further MCs in specific cerebral region but the duration of acute stress itself does not affect the activation level.


Stress duration; mast cell; thalamus; hippocampus

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McEwen B. Physiology and Neurobiology of Stress and Adaptation: Central Role of the Brain. Physiological Reviews. 2007;87(3):873904.

Work related Stress, Anxiety and Depression Statistics in Great Britain 2016 [Internet]. HSE. 2016 [cited 10 November 2016]. Available

from: http://www.hse.gov.uk/statistics/causdis/stress/stress.pdf

Glaser R, Kiecolt-Glaser J. How stress damages immune system and health. Discov Med. 2016;5(26):165-9.

Nautiyal K. Mast cells affect brain physiology and behavior [Internet]. Colombia Academic Commons. 2011 [cited 10 November 2016].

Available from: https://academiccommons.columbia.edu/catalog/ac%3A131582

Pang X, Letourneau R, Rozniecki J, Wang L, Theoharides T. Definitive characterization of rat hypothalamic mast cells. Neuroscience. 1996;73(3):889-902.

Ringvall M, Rönnberg E, Wernersson S, Duelli A, Henningsson F, Åbrink M et al. Serotonin and histamine storage in mast cell secretory granules is dependent on serglycin proteoglycan. Journal of Allergy and Clinical Immunology. 2008;121(4):1020-1026.

Li W, Guo T, Liang D, Sun Y, Kingery W, Clark J. Substance P Signaling Controls Mast Cell Activation, Degranulation, and Nociceptive Sensitization in a Rat Fracture Model of Complex Regional Pain Syndrome. Anesthesiology. 2012;116(4):882-895.

Theoharides T, Spanos C, Pang X, Alferes L, Ligris K, Letourneau R et al. Stress-induced intracranial mast cell degranulation: a corticotropin-releasing hormone-mediated effect. Endocrinology. 1995;136(12):57455750.

Belda X, Fuentes S, Daviu N, Nadal R, Armario A. Stress-induced sensitization: the hypothalamic–pituitary–adrenal axis and beyond. Stress. 2015;18(3):269-279.

Dhabhar F. Enhancing versus Suppressive Effects of Stress on Immune Function: Implications for Immunoprotection and Immunopathology. Neuroimmunomodulation. 2009; 16 (5): 300317.

Daeng B, Paundralingga O, Widodo A, Sujuti H, Mintaroem K, Widjajanto E. Cite a Website - Cite This For Me [Internet]. Aseanjournalofpsychiatry.org. 2016 [cited 10 November 2016]. Available from: http://aseanjournalofpsychiatry.org/index.php/aseanjournalofpsychiatry/article/download/367/242

Alysandratos K, Asadi S, Angelidou A, Zhang B, Sismanopoulos N, Yang H et al. Neurotensin and CRH Interactions Augment Human Mast Cell Activation. PLoS ONE. 2012;7(11):e48934.

Nautiyal K, Dailey C, Jahn J, Rodriquez E, Son N, Sweedler J et al. Serotonin of mast cell origin contributes to hippocampal function. European Journal of Neuroscience. 2012;36(3):2347-2359.

Zarrindast M, Malekmohamadi N, Tabaei S, Ahmadi S. Intracerebroventricular Injection of Histamine Induces State-Dependency through H1 Receptors. Iranian Journal of Pharmaceutical Research. 2006;4:255-260.

Alvarez E. Histaminergic systems of the limbic complex on learning and motivation. Behavioural Brain Research. 2001;124(2):195202.

Henckens M, Hermans E, Pu Z, Joels M, Fernandez G. Stressed Memories: How Acute Stress Affects Memory Formation in Humans. Journal of Neuroscience. 2009;29(32):1011110119.

Esposito P, Gheorghe D, Kandere K, Pang X, Connolly R, Jacobson S et al. Acute stress increases permeability of the blood–brainbarrier through activation of brain mast cells. Brain Research. 2001;888(1):117-127.

Theoharides T, Rozniecki J, Sahagian G, Jocobson S, Kempuraj D, Conti P et al. Impact of stress and mast cells on brain metastases. Journal of Neuroimmunology. 2008;205(12):1-7.

Rozniecki J, Sahagian G, Kempuraj D, Tao K, Jocobson S, Zhang B et al. Brain metastases of mouse mammary adenocarcinoma is increased by acute stress. Brain Research. 2010;1366:204-210.

Coutinho A, Brown J, Yang F, Brownstein D, Gray M, Seckl J et al. Mast Cells Express 11βhydroxysteroid Dehydrogenase Type 1: A Role in Restraining Mast Cell Degranulation. PLoS ONE. 2013;8(1):e54640.

Bhattacharya SBhattacharya D. Effect of restraint stress on rat brain serotonin. Journal of Biosciences. 1982;4(3):269-274.

Bugajski A, Chłap Z, Gądek-Michalska, Bugajski J. Effect of isolation stress on brain mast cells and brain histamine levels in rats. Agents and Actions. 1994; 41 (S1) : C75 - C76.


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