NEURAL PAIN PATHWAY TRACING OF RABBIT ISCHEMIC HEART BY DOUBLE-RETROGRADE NEUROTRACING
Keywords:myocardial ischaemia, neurotracing, referred pain
AbstractBackground. Myocardial ischaemiaÂ leads to angina pectoris or referred pain, whichhappens because of theÂ inability of the brain to distinguish the visceral afferent inputs from the somatic afferent inputs since theyÂ run along a common pathway via the dorsal root ganglia.
Objective. To distinguish specific areas of the rabbit heart that are projected to specific dorsalÂ root ganglia, which then associates to its specific dermatomes.
Methods. A double-retrograde neurotracing method was used, with True Blue and Nuclear Yellow as theÂ neurotracers. Rabbits were divided into 3 groups, which the first and second groups were ligated at theÂ left anterior descending artery and at the left circumflex artery, respectively.The third group acted as theÂ control group, without ligation.
Results. There is significant association between the site of ligation to the projection of the neurotracers atÂ specific dorsal root ganglia (p<0.05). The first group showed high tendency to be projected to T2 and theÂ second group showed a high tendency to project to T1.
Conclusion. This study shows that the rabbit heart can be specifically projected neuronally to specificÂ dorsal root ganglia, following coronary artery ligation.
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