DOES THE COMBINATION OF ROSMARINUS OFFICINALIS AND CENTELLA ASIATICA NANOEMULSIONS AFFECT BDNF EXPRESSION IN THE GESTATIONAL DIABETES MELLITUS MODEL IN ZEBRAFISH LARVAE
DOI:
https://doi.org/10.21776/ub.mnj.2024.010.02.08Keywords:
GDM, PEPCK, BDNF, Rosmarinus officinalis, Centella asiatica, nanoemulsionsAbstract
Background: Pancreatic β-cells compensate for the increased insulin demand due to increasing glucose levels during pregnancy or in gestational diabetes mellitus (GDM). The enzyme that regulates the rate of gluconeogenesis production is PEPCK. Circulatory and metabolic disturbances in the brain caused by hyperglycemia could affect brain-derived neurotrophic factor (BDNF) expression as an agent for neurodevelopment.
Objective: The purpose of this work is to ascertain the impact of combining nanoemulsions of Rosmarinus officinalis and Centella asiatica on the expression of BDNF and PEPCK.
Methods: This study was a true experimental laboratory using zebrafish larvae. The larvae were exposed to 3% glucose and a combination of nanoemulsion extracts. The study sample of zebrafish larvae at 72 hpf (hour post fertilization) was divided into control, glucose, treatment 1, 2, and 3, with the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 2.5 μg/ml, 5 μg/ml, and 10 μg/ml, respectively. PEPCK and BDNF expression were measured by the real-time PCR method.
Results: The expression of PEPCK increased significantly in the glucose group (3.05 ± 0.27). The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly decreased PEPCK (p-value 0,001 < 0.05). The BDNF level did not significantly differ (p-value 0.253 >0.05) among groups, but the highest level (1.06 ± 0.60) was gained in the T2 group, which received an embryonic medium (EM), 3% glucose, and the combination of Rosmarinus officinalis and Centella asiatica nanoemulsions at 5μg/ml.
Conclusion: The combination of Rosmarinus officinalis and Centella asiatica nanoemulsions significantly reduces PEPCK expression but does not affect BDNF expression.
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References
PERKENI (Persatuan Endokrinologi Indonesia). Pedoman diagnosis dan penatalksanaan hiperglikemia dalam kehamilan 2021. PB. PERKENI; 2021.
Abell SK, De Courten B, Boyle JA, Teede HJ. Inflammatory and other biomarkers: Role in pathophysiology and prediction of gestational diabetes mellitus. Int J Mol Sci; 2015. 16(6):13442–73.
DOI: 10.3390/ijms160613442
Khotimah H, Safira NAP, Anindita D, Weningtyas A, Prima WE, Kalsum U. Ethanolic extract of Salacca
zalacca peel reduce IL-1β and apoptosis in high glucose induced zebrafish embryo. GSC Biol Pharm Sci; 2021. 16(3):024–33. DOI: 10.30574/gscbps.2021.16.3.0213
Newsholme P, Cruzat VF, Keane KN, Carlessi R, De Bittencourt PIH. Molecular mechanisms of ROS production and oxidative stress in diabetes. Biochem J; 2016. 473(24):4527–50. DOI: 10.1042/BCJ20160503C
Fatimah RN. Diabetes melitus tipe 2. Major J; 2015. 4(Diabetes Melitus Tipe 2):93–101.
DOI: 10.25026/jsk.v5i5.1896
Annis F, Purnomo H, Balafif F. The effect of propolis extract administration on apoptosis through brain-derived neurotropic factor. J Kedokt Brawijaya; 2017. 29(3):209–15. DOI: 10.21776/ub.jkb.2017.029.03.5
Schmidt R, Strähle U, Scholpp S. Neurogenesis in zebrafish - from embryo to adult. Neural Dev; 2013. 8(1):1–13. DOI: 10.1186/1749-8104-8-3
Diotel N, Lübke L, Strähle U, Rastegar S, Morales-garcia JA. Common and distinct features of adult neurogenesis and regeneration in the telencephalon of zebrafish and mammals; 2020. 14(September):1–22. DOI: 10.3389/fnins.2020.568930
Shahdevi Nandar Kurniawan, Zamroni Afif YQM. Terapi antioksidan pada neuropati diabetes- google buku [Internet]. Universitas Brawijaya Press; 2021. [quoted on 14 Desember 2022]. Available from: https://books.google.co.id/books =kandungan+neurotropik+pada+rosmarinus+officinalis
Sánchez-Camargo A del P, Herrero M. Rosemary (Rosmarinus officinalis) as a functional ingredient: Recent scientific evidence. Curr Opin Food Sci [Internet]; 2017. 14:13–9.
DOI: 10.1016/j.cofs.2016.12.003
Irsalina A. Efek daun pegagan (C. asiatica) sebagai neuroprotektor. Eksakta J Penelit dan Pembelajaran MIPA; 2020. 5(2):226.
DOI: 10.31604/eksakta.v5i2.226-231
Puji, Nur Nabilah Ahmad, Lokanathan Yogeswaran, Idrus R. The effect of Centella asiatica (L.) urban on the organotypic model of spinal cord injury. Sains Malysiana [Internet]; 2018. 47(11):2783–8. Available from: http://www.ukm.my/jsm/english_journals/vol47num11_2018/vol47num11_2018pg2783-2788.htm
Bhatt H, Saklani S, Upadhayay K. Anti-oxidant and anti-diabetic activities of ethanolic extract of Primula Denticulata Flowers. Indones J Pharm; 2016. 27(2):74–9. DOI: 10.14499/indonesianjpharm27iss2pp74
Jusril NA, Abu Bakar SI, Khalil KA, Md Saad WM, Wen NK, Adenan MI. Development and optimization of nanoemulsion from ethanolic extract of Centella asiatica (NanoSECA) using d-optimal mixture design to improve blood-brain barrier permeability. Evidence-based Complement Altern Med; 2022.
DOI: 10.1155/2022/3483511
Diekmann H, Anichtchik O, Fleming A, Futter M, Goldsmith P, Roach A, et al. Decreased BDNF levels are a major contributor to the embryonic phenotype of huntingtin knockdown zebrafish. J Neurosci; 2009. 29(5):1343-9. DOI: 10.1523/JNEUROSCI.6039-08.2009
Khotimah H, Dewi Lestari Ismail D, Widasmara D, Riawan W, Retnaningtyas E, Weka Nugraheni R, et al. Ameliorative effect of gel combination of Centella asiatica extract transfersomes and rosemary essential oil nanoemulsion against UVB-induced skin aging in Balb/c mice. F1000Research; 2022. 11:288. Available from: https://f1000research.com/articles/11-288
Chiefari E, Arcidiacono B, Foti D, Brunetti A. Gestational diabetes mellitus: An updated overview. J Endocrinol Invest; 2017. 40(9):899–909.
DOI: 10.1007/s40618-016-0607-5
Rizwan H, Pal S, Sabnam S, Pal A. High glucose augments ROS generation regulates mitochondrial dysfunction and apoptosis via stress signalling cascades in keratinocytes. Life Sci [Internet]; 2020. 241:117148. DOI: 10.1016/j.lfs.2019.117148
Carnovali M, Luzi L, Banfi G, Mariotti M. Chronic hyperglycemia affects bone metabolism in adult zebrafish scale model. Endocrine; 2016. 54(3):808–17. DOI: 10.1007/s12020-016-1106-3
Singh A, Castillo HA, Brown J, Kaslin J, Dwyer KM, Gibert Y. High glucose levels affect retinal patterning during zebrafish embryogenesis. Sci Rep [Internet]; 2019. 9(1):1–13. DOI: 10.1038/s41598-019-41009-3
Bao TQ, Li Y, Qu C, Zheng ZG, Yang H, Li P. Antidiabetic effects and mechanisms of rosemary (Rosmarinus officinalis L.) and its phenolic components. Am J Chin Med; 2020. 48(6):1353–68. DOI: 10.1142/S0192415X20500664
Tu Z, Moss-Pierce T, Ford P, Jiang TA. Rosemary (Rosmarinus officinalis L.) extract regulates glucose and lipid metabolism by activating AMPK and PPAR pathways in HepG2 cells. J Agric Food Chem; 2013. 61(11):2803–10. DOI: 10.1021/jf400298c
Banjarnahor E, Wangko S. Sel beta pankreas sintesis dan sekresi insulin. J Biomedik; 2013. 4(3).
DOI: 10.35790/jbm.4.3.2012.795
Masola B, Oguntibeju OO, Oyenihi AB. Centella asiatica ameliorates diabetes-induced stress in rat tissues via influences on antioxidants and inflammatory cytokines. Biomed Pharmacother [Internet]; 2018. 101(October 2017):447–57.
DOI: 10.1016/j.biopha.2018.02.115
Ding S, Anton N, Akram S, Er-Rafik M, Anton H, Klymchenko A, et al. A new method for the formulation of double nanoemulsions. Soft Matter; 2017. 13(8):1660–9. Available from: https://pubs.rsc.org/en/content/articlelanding/2017/sm/c6sm02603f
Rita CI. Pengaruh kombinasi transfersom pegagan dan nanoemulsi rosemary terhadap ekspresi transforming growth factor beta dan matrix metalloproteinase-9 pada proses penyembuhan luka insisi tikus (Rattus norvegicus). Universitas Brawijaya; 2020.
DOI: 10.31964/mltj.v9i2.537
Klein AB, Williamson R, Santini MA, Clemmensen C, Ettrup A, Rios M, et al. Blood BDNF concentrations reflect brain-tissue BDNF levels across species. Int J Neuropsychopharmacol; 2011. 14(3):347–53.
DOI: 10.1017/S1461145710000738
Mudjihartini N. Brain-derived neurotrophic factor (BDNF) dan proses penuaan: Sebuah tinjauan. J Biomedika dan Kesehat; 2021. 4(3):120–9.
DOI: 10.18051/JBiomedKes.2021.v4.120-129
Wu SY, Pan BS, Tsai SF, Chiang YT, Huang BM, Mo FE, et al. BDNF reverses aging-related microglial activation. J Neuroinflammation; 2020. 17(1):1–18. DOI: 10.1186/s12974-020-01887-1
Moosaie F, Mohammadi S, Saghazadeh A, Firouzabadi FD, Rezaei N. Brain-derived neurotrophic factor in diabetes mellitus: A systematic review and meta-analysis. PLoS One [Internet]; 2023. 18(2 February):1–23. DOI: 10.1371/journal.pone.0268816
Negrón AL, Beymer M, Yu G, Warren KA, Acosta-Martínez M. Prolonged hyperglycemia & hyperinsulinemia increases BDNF mRNA expression in the posterior ventromedial hypothalamus and the dorsomedial hypothalamus of fed female rats. Neuroscience; 2015. 303(July):422–32.
DOI: 10.1016/j.neuroscience.2015.07.018
Hashim P, Sidek H, Helan MHM, Sabery A, Palanisamy UD, Ilham M. Triterpene composition and bioactivities of centella asiatica. Molecules; 2011. 16(2):1310–22. DOI: 10.3390/molecules16021310
Soumyanath A, Zhong YP, Henson E, Wadsworth T, Bishop J, Gold BG, et al. Centella asiatica extract improves behavioral deficits in a mouse model of Alzheimer’s disease: Investigation of a possible mechanism of action. Int J Alzheimers Dis; 2012. 2012. DOI: 10.1155/2012/381974
Maulida U, Jofrishal, Mauliza. Uji aktivitas antidiabetes ekstrak etanol pada tanaman pegegan (Centella asiatica (L) Urban. KATALIS J Pendidik Kim dan Ilmu Kim; 2019. 2(2):1–8. Available from: https://ejurnalunsam.id/index.php/katalis/article/view/1833
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