LIGHT EXPOSURE’S EFFECTS ON INACTIVE STATE DURATION AND SLEEP LATENCY IN ZEBRAFISH (DANIO RERIO) LARVAE INSOMNIA MODEL

Authors

  • Zamroni Afif Doctoral Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Mochammad Istiadjid Eddy Santoso Doctoral Program, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Husnul Khotimah Departement of Pharmacology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Irawan Satriotomo Indonesian Neuroscience Institute, Indonesia
  • Edi Widjajanto Department of Clinical Pathology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Masruroh Rahayu Department of Neurology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Shahdevi Nandar Kurniawan Department of Neurology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Dheka Sapti Iskandar Master Program Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Annisatul Hakimah Master Program Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Syafiatul Azizah Master Program Biomedical Science, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Nurvia Andriani Residency Program, Department of Neurology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia
  • Kartika Agustina Residency Program, Department of Neurology, Faculty of Medicine, Universitas Brawijaya, Malang, Indonesia

DOI:

https://doi.org/10.21776/ub.mnj.2022.008.02.11

Keywords:

Insomnia, zebrafish, inactive state duration and sleep latency

Abstract

Background: Insomnia is defined as difficulty falling or staying asleep or a sleep state that cannot restore the body's condition. The zebrafish (Danio rerio) is a vertebrate model that has been extensively studied to study sleep and neurological disorders. One of the most widely used methods to examine the effect of the light-dark cycle on the circadian system is by exposing animals and humans to pulse wave light. Objective: To see the effect of light exposure on zebrafish larvae by looking at inactive state duration and sleep latency in zebrafish (Danio rerio) larvae insomnia model. Methods: This study used four groups of zebrafish larvae i.e : (1) normal group (2) minutes of light exposure and 2 minutes off (2/2)), (3) Four minutes of light exposure and 1 minute off (4/1), and (4)  24 hours on (24/0). Observation of larval movement was carried out on 5, 6, and 7 dpf (day post fertilization). Observation time was 30 minutes before and after turned off of light exposure. Results: There were significant differences on days 5, 6, and 7 between the normal group and the three light treatment groups on inactive state duration and sleep latency in dark and light conditions with p-values (p<0.05) and (p< 0.031), but there was no significant difference among groups of light exposure 2 minutes on 2 minutes off, 4 minutes on 1 minute off, and 24 hours on. The 24-hour on treatment showed the most inactive state duration among the light treatments, while the sleep latency was found in the 24-hour treatment. Conclusion: Light treatment of 2 minutes on 2 minutes off, 4 minutes on 1 minute off, and 24 hours on can cause insomnia, but the most substantial insomnia effect is obtained from the 24-hour treatment.

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Published

2022-07-06

How to Cite

Afif, Z., Santoso, M. I. E. ., Khotimah, H. ., Satriotomo, I. ., Widjajanto, E. ., Rahayu, M. ., Kurniawan, S. N. ., Iskandar, D. S., Hakimah, A. ., Azizah, S. ., Andriani, N. ., & Agustina, K. . (2022). LIGHT EXPOSURE’S EFFECTS ON INACTIVE STATE DURATION AND SLEEP LATENCY IN ZEBRAFISH (DANIO RERIO) LARVAE INSOMNIA MODEL. Malang Neurology Journal, 8(2), 129–134. https://doi.org/10.21776/ub.mnj.2022.008.02.11

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Research Article