THE EFFECT OF INFECTION OF MYCOBACTERIUM TUBERCULOSIS STRAIN H37RV TOWARDS THE EXPRESSION OF TNF- α IN THE BRAIN
Keywords:
TNF-α, Mycobacterium tuberculosis, brain, imunohistokimiaAbstract
Background. Tuberculosis caused by mycobacterium tuberculosis. The support of these inflamated factors contained in this focus determine the prognosis of TB towards Central Nervous System. One of the inflammation factors is TNF-α.Objective. To find the expression of TNF-α on the brain which are infected by M. tuberculosis.
Methods. Experimental study, Wild Mus musculus to compare the group which has got infected before. The similiar study has been conducted by Laksmi Wulandari, pulmonologist. The observation towards the expression of TNF-a into a mouse’s brain was done by using the imunohistokimia method. It is marked by the brown color in the core, sitoplasma and the cell with upgrading size 100x times 20.
Results. This is evident that significanly corelation (P = 0.000) between independent Mycobacterium tuberculosis infection at incubation periode 8 and 16 weeks) and dependent variabel (TNF-α expression on brain winstar tissue that infected by Mycobacterium tuberculosis for 8 and 16 weeks).
Conclusion. Strong relationship between expression of TNF-a and the M. Tuberculosis’ infection.
References
Handayani, S. Respon Imunitas Seluler pada Infeksi Tuberkulosis Paru. 2008. Cermin Dunia Kedokteran dalam www._kalbe_com.
Coppack, S.W. Pro-Inflammatory cytokines and adipose tissue.Proceedings of Nutrition Society. 2001. 60:349-356.
Djuwita, Ita. Induksi Ekstrak Pegagan Secara in vitro terhadap Proliferasi dan Diferensiasi Sel-Sel Otak Besar Anak Tikus. Jurnal Veteriner Juni 2013 Vol. 14 No. 2: 138-144
Gary, Davin S, Annadora J Bruce-Keller, Mark S Kindy and Mark P Mattson. Ischemic and Excitotoxic Brain Injury Is Enhanced in Mice Lacking the p55 Tumor Necrosis Factor Receptor. 2000.
Rock R. Bryan., et.all. Central Nervous System Tuberculosis: Pathogenesis and Clinical Aspects, CLIN. MICROBIOL 2008. (21). 2.P:243361.
Skoog, T., Dichtl, W.,Boquist, S., Andersson, S.,Karpe, F.,Tang, R.,Bond, M.G.,Faire, U., Nilsson, J., Eriksson, P dan Hamsten, A. Plasma Tumour Necrosis Factor and Early Carotid Atherosclerosis in Healthy Middle Aged Men. Eur Heart J. 2002. 23:376-383.
Sukhanov, S.,Higashi, Y., Yung Shai, S., Vaughn, C., Mohler, J., Li,Y., Hua Song, Y.,Titterington, P. IGF-1 Reduces Inflammatory Responses, suppresses Oxidative Stress, and Decreases Atheroschlerosis Progresion in ApoE-Deficient Mice. Arterioscler Thromb Vasc Biol. 2007. 27;84-2690.
Tsenova, Liana, Amy Bergtold, Victoria H. Freedman, Richard A. Young, and Gilla Kaplan. Tumor necrosis factor α is a determinant of pathogenesis and disease progression in mycobacterial infection in the central nervous system. 2000.
World Health Organization. Global tuberculosis control. In: World Health Organization report 2012. Geneva: World Health Organisation; 2011. p.1–258.
