Muhamad Rasjad Indra, Zanella Yolanda Lie
  MNJ, pp. 5-11  


Background. Grape peel and seed extract (Vitis vinifera), that has resveratrol, is one of many antioxidants that can pass through blood brain barrier and can induce release neurotrophic factor that contribute in ERK 1/2 pathway mechanism in post stroke.
Objective. To prove that grape peel and seed extract can regenerate neuron in brain functional.
Methods. True experimental design with five groups in this research. The five groups are negative control, positive control, grape peel and seed extract 50mg/KgBW, 100mg/KgBW, and 200mg/KgBW. rats are given grape peel and seed extract in variable dose to know how extract’s effect in neuron repairment. The repairment is monitored from ladder rung walking test score.
Results. Range average score ladder rung walking test post stroke dan post treatment group N, K, Ra, Rb, dan Rc, were 0 ± 0, 0.001028933 ± 0.011664445, 0.123214286 ± 0.019834983, 0.064744427 ± 0.024296721, 0.03781401 ± 0.006888803. Statistical test used Annova significantly p;0,001.Dose 50mg/KgBW is effective in repairing neuron.
Conclusion. Grape’s leather and Seed extract 50 mg/kgBW can improve neuron regeneration on animal model.


Stroke; grape; resveratrol; ladder rung walking test

Full Text:



Cruz-Flores,S., Rabinstein A, Biller J, Elkind MS, Griffith P, Gorelick PB, Howard G, Leira EC, Morgenstern LB, Ovbiagele B, Peterson E, Rosamond W, Trimble B, Valderrama AL. Racial-ethnic disparities in stroke care: the American experience: a statement for healthcare professionals from the American Heart Association. American Stroke Association.Stroke. 2006. 42(7): 2091-116.

Roger, V.L., et al. Heart disease and stroke statistics-2012 update: a report from the American Heart Association. Circulation. 2012. 125(1):e2-220.

World Health Organization. The International Agenda for Stroke Marc Fisher, MD, University of Massachussets. Stroke AHA/ ASA. Norrving B. 1st Global Conferences on Healthy Lifestyles and Noncommunicable Diseases Control. Moscow. 2011.

Riset Kesehatan Dasar. 2007. Jakarta: Badan Penelitian dan Pengembangan Kesehatan, Departemen Kesehatan, Republik Indonesia.

Yayasan Stroke Indonesia. Jakarta, Republik Indonesia. 2011.

Zieve, D: Stroke.Neurosurgery, Cedars-Sinai Medical Center.National Library of Medicine. Los Angeles. 2011.

Caplan, A: Adult mesenchymal stem cells for tissue engineering versus regenerative medicine. J cell physiol. 2007, 213(2): 341347.

Soyuer, F. Ischemic Stroke: Motor Impairment and Disability With Relation To Age and Lesion Location. The Internet Journal of Neurology. 2005. 3(2): DOI: 10.5580/9b4.

Chamorro, A., Marshall, R.S., Sole, J.V., Tolosa, E., Mohr, J.P. Motor Behavior in Stroke Patients With Isolated Medial Frontal Ischemic Infarction, American Heart Association, 1997, 28: 1755-1760.

Cavanaugh, J., Lin, E., Leak, R.K., Perez, R.G., Zigmond, M.J: Rapid activation of ERK by 6hydroxydopamine promotes survival of dopaminergic cells. Journal Neurosci Res. 2008, 86(1):108-117.

Flaster, M. How to Minimize Damage from Stroke. Diakses pada tanggal 13 Oktober 2012.

Mayo Clinic: Stroke: Treatment and Drug. Diakses pada tanggal 30 Oktober 2012

Hennekens, C.H., Dyken, M. L., Aspirin as a Therapeutic Agent in Cardiovascular Disease. 1997.

Rist, P. M. Effect of Low-Dose Aspirin on Functional Outcome from Cerebral Vascular Events in Women. Stroke, 2013; 44: 432-436.

Wang YF, Tsirka SE, Strickland S, Stieg PE, Soriano SG, Lipton SA. Tissue plasminogen activator (tPA) increases neuronal damage after focal cerebral ischemia in wild-type and tPA-deficient mice. Nat Med 1998. 4:228-231

Bastianetto, S. Quirion, R: Resveratrol and red wine constituents: evaluation of their neuroprotective properties.Pharm. News. 2001, (8):33–38.

Wang, Y.J., He, F, Li X.L: The neuroprotection of resveratrol in the experimental cerebral ischemia. Department of Neurology, Tiantan Hospital, Capital University of Medical Sciences, Beijing. Europe PubMed Central. 2003; 83(7):534-536.

Zhang, F., Lu, Y.F., Wu, Q., Liu, J., Shi, J.S. Resveratrol promotes neurotrophic factor release from astroglia. Exp Bio Med, 2012; 237(8): 943-948.

Perez, A.L.R., Reventos, L., Lacueva, C.A., Boronat, C.T. Method for the Quantitative Extraction of Resveratrol and Piceid Isomers in Grape Berry Skins. Effect of Powdery Mildew on the Stilbene Content, J. Agric. Food Chem. 2001, 49, 210-215.

Xiang Y, Zhang T, Zhang X, Ma L. [Quantitative analysis of resveratrol from grape seeds and grape skins by high performance liquid chromatography method]. 2003, Wei Sheng Yan Jiu. 2003 Sep; 32(5):490-2.

Jalil, EC et al. Resveratrol potently reduces prostaglandin E2 production and free radical formation in lipopolysaccharide-activated primary rat microglia. Journal of Neuroinflammation. 2007. 4:25. DOI:10.1186/1742-2094-4-25.

Murwani, S., Mulyohadi, A., Ketut, M et al. Diet aterogenik pada tikus putih (Rattus novergicus strain Wistar) sebagai model hewan aterosklerosis, Jurnal Kedokteran Brawijaya, 2006, Vol.22, No.1, p6-9.

Schaar, K.L., Brenneman, M.M., Savitz, S.I. Functional Assessments in the Rodent Stroke Model. Experimental & Transitional Stroke Medicine. 2010. 2:13.


  • There are currently no refbacks.