HISTOLOGICAL ANALYSIS OF WISTAR RATS SCIATIC NERVES SUBJECTED TO COMPRESSION AND TREATED WITH LOW LEVEL LASER THERAPY AND NEURAL MOBILIZATION
Keywords:
Nerve Regeneration, Sciatic Nerve, Physical Therapy ModalitiesAbstract
Background: The physical therapy resources, as Low-Level Laser Therapy (LLLT) and neural mobilization are usually used with regenerative purpose and for the relief of symptoms for peripheral nerve injury. However, there is a lack of consensus on its effects as well as its association.
Objective: Evaluate the influence of low-level laser therapy (LLLT) associated with neural mobilization (NM) on nerve regeneration of female Wistar rats subjected to sciatic compression.
Methods: Twenty four Wistar rats were divided into: G1 (Placebo), G2 (NM), G3 (LLLT) and G4 (NM+LLLT), all animals were subject to compression of the right sciatic nerve. The treatment occurred on the 3rd, 5th, 7th, 10th, 12th and 14th postoperative day (PO). On the 17th PO the animals were euthanized. Histological and morphometric analysis of the right (compression) and the left (control) sciatic nerve were performed.
Results: In the morphometric analysis no significant differences between groups, but differences among sides were found. Histological analysis revealed the presence of inflammatory infiltrate and disruption of nerve fibers in the right sciatic nerves.
Conclusion: Both techniques used (LLLT and neural mobilization), isolated and associated, on the parameters proposed, were not effective to accelerate the process of nerve regeneration.
References
Lewis R, Williams N, Matar HE, Din N, Fitzsimmons D, Phillips C, et al. The clinical effectiveness and cost- effectiveness of management strategies for sciatica: systematic review and economic model. Health Technol Assess (Rockv); 2011.15(39):1–578. DOI: 10.3310/hta15390
Ailianou A, Fitsiori A, Syrogiannopouou A, Toso S, Viallon M, Merlini L, et al. Review of the principal extra spinal pathologies causing sciatica and new mri approaches. Br J Radiol; 2012.85(1014):672–81. DOI: 10.1259/bjr/84443179
Ashworth J, Konstantinou K, Dunn KM. Prognostic factors in non-surgically treated sciatica: a systematic review. BMC Musculoskelet Disord; 2011.12. DOI: 10.1186/1471-2474-12-208
Kristianto H, Mardiati NPJ. The effects of earthworms’ (pheretima aspergillum) ethanol extract toward the improvement of nerve fibers density in diabetic ulcers care degree ii of rats. Malang Neurology Journal; 2017.3:61–72. DOI: 10.21776/ub.mnj.2017.003.02.3
Malshikare VA, Desai SM, Arekar AS, Bhosale NA, Bonde SR, Awadhani MA. Comparison of Carpastretch® with splint in non-surgical treatment of carpal tunnel syndrome: a randomized open label study. Malang Neurology Journal; 2019.5:14–20. DOI: 10.21776/ub.mnj.2019.005.01.3
Gonçalves RB, Marques JC, Monte-raso VV, Zamarioli A, Carvalho LC, Fazan VPS, et al. Efeitos da aplicação do laser de baixa potência na regeneração do nervo isquiático de ratos /Effects of low-power laser on injured rat sciatic nerve regeneration. Fisioter Pesqui; 2010.17(1):34–9. DOI: 10.1590/S180929502010000100007
Silva LI, Rocha BP, Antunes JS, Karvat J, Kakihata CMM, Mattjie TF, et al. Evaluation of the pressure pain threshold after neural mobilization in individuals with sciatica. Eur J Physiother; 2013.15(July):146–50. DOI: 10.3109/21679169.2013.831119
Udina E, Puigdemasa A, Navarro X. Passive and active exercise improve regeneration and muscle reinnervation after peripheral nerve injury in the rat. Muscle Nerve; 2011.43(4):500–9. DOI: 10.1002/mus.21912
Alves ACA, Carvalho P de TC de, Parente M, Xavier M, Frigo L, Aimbire F, et al. Low-Level laser therapy in different stages of rheumatoid arthritis: a histological study. Lasers Med Sci; 2013.28(2):529–36. DOI: 10.1007/s10103-012-1102-7
Reis FA, Belchior ACG, Carvalho P de TC de, Silva BAK da, Pereira DM, Silva IS, et al. Effect of laser therapy (660 nm) on recovery of the sciatic nerve in rats after injury through neurotmesis followed by epineural anastomosis. Lasers Med Sci; 2009.24(5):741–7. DOI: 10.1007/s10103-008-0634-3
Dias FJ, Issa JPM, Coutinho-Netto J, Fazan VPS, Sousa LG, Iyomasa MM, et al. Morphometric and high resolution scanning electron microscopy analysis of low-level laser therapy and latex protein (hevea brasiliensis) administration following a crush injury of the sciatic nerve in rats. J Neurol Sci; 2015.349(1–2):129–37. DOI: 10.1016/j.jns.2014.12.043
Huisstede BM, Hoogvliet P, Franke TP, Randsdorp MS, Koes BW. Carpal tunnel syndrome: effectiveness of physical therapy and electrophysical modalities. an updated systematic review of randomized controlled trials. Arch Phys Med Rehabil; 2018.99(8):1623–1634.e23. DOI: 10.1016/j.apmr.2017.08.482
Chen YJ, Wang YH, Wang CZ, Ho ML, Kuo PL, Huang MH, et al. Effect of low level laser therapy on chronic compression of the dorsal root ganglion. PLoS One; 2014.9(3):e89894. DOI: 10.1371/journal.pone.0089894
Barez MM, Tajziehchi M, Heidari MH, Bushehri A, Moayer F, Mansouri N, et al. Stimulation effect of low level laser therapy on sciatic nerve regeneration in rat. J Lasers Med Sci; 2017.8(Suppl 1):S32–7. DOI: 10.15171/jlms.2017.s7
Oliveira Jr HF de, Teixeira ÃH. Mobilização do sistema nervoso: avaliação e tratamento /Mobilization nervous system: assessment and treatment. Fisioter em Mov; 2007.20(3):41–53. Available from: https://periodicos.pucpr.br/index.php/fisio/article/view/18911/18289
Castilho J, Ferreira LAB, Pereira WM, Neto HP, Morelli JG da S, Brandalize D, et al. Analysis of electromyographic activity in spastic biceps brachii muscle following neural mobilization. J Bodyw Mov Ther; 2012.16(3):364–8. DOI: 10.1016/j.jbmt.2011.12.003
Beneciuk JM, Bishop MD, George SZ. Effects of upper extremity neural mobilization on thermal pain sensitivity: a sham-controlled study in asymptomatic participants. J Orthop Sport Phys Ther; 2009.39(6):428–38. DOI: 10.2519/jospt.2009.2954
Véras LST, Vale RG de S, Mello DB de, Castro JAF de, Lima V, Trott A, et al. Electromyography function, disability degree, and pain in leprosy patients undergoing neural mobilization treatment. Rev Soc Bras Med Trop; 2012.45(1):83–8. DOI: http://dx.doi.org/10.1590/S0037-86822012000100016
Bennett GJ, Xie YK. A peripheral mononeuropathy in rat that produces disorders of pain sensation like those seen in man. Pain; 1988.33(1):87–107. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/2837713
Karvat J, Antunes JS, Bernardino GR, Kakihata CMM, Bertolini GRF. Effect of low-level laser and neural mobilization on nociceptive threshold in experimental sciatica. Rev Dor. 2014;15(3):207–10. DOI: 10.5935/1806-0013.20140045
Geuna S, Tos P, Guglielmone R, Battiston B, Giacobini-robecchi MG. Methodological issues in size estimation of myelinated nerve fibers in peripheral nerves. Anat Embryol; 2001.204(1):1–10. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/11506429
Sousa FF de A, Ribeiro TL, Fazan VPS, Barbieri CH. Lack of effectiveness of laser therapy applied to the nerve course and the correspondent medullary roots. Acta Ortop Bras; 2013.21(2):92–7. DOI: 10.1590/S1413-78522013000200005
Alcântara CC, Gigo-benato D, Salvini TF, Oliveira ALR, Anders JJ, Russo TL. Effect of low-level laser therapy (lllt) on acute neural recovery and inflammation-related gene expression after crush injury in rat sciatic nerve. Lasers Surg Med; 2013.45(4):246–52. DOI: 10.1002/lsm.22129
Endo C, Barbieri CH, Mazzer N, Fasan VS. LowPower laser therapy accelerates peripheral nerves’ regeneration. Acta Ortop Bras; 2008.16(5):305–10. DOI: 10.1590/S1413-78522008000500011
Martins DF, Mazzardo-martins L, Gadotti VM, Nascimento FP, Lima DAN, Speckhann B, et al. Ankle joint mobilization reduces axonotmesis-induced neuropathic pain and glial activation in the spinal cord and enhances nerve regeneration in rats. Pain; 2011.152(11):2653–61. DOI: 10.1016/j.pain.2011.08.014
Marcioli MAR, Coradini JG, Kunz RI, Ribeiro LDFC, Brancalhão RMC, Bertolini GRF. Nociceptive and histomorphometric evaluation of neural mobilization in experimental injury of the median nerve. ScientificWorldJournal; 2013. DOI: 10.1155/2013/476890
De-la-Llave-Rincon AI, Ortega-Santiago R, AmbiteQuesada S, Gil-Crujera A, Puentedura EJ, Valenza MC, et al. Response of pain intensity to soft tissue mobilization and neurodynamic technique: a series of 18 patients with chronic carpal tunnel syndrome. J Manip Physiol Ther; 2012;35(6):420–7. DOI: 10.1016/j.jmpt.2012.06.002
Oskay D, Meriç A, Kirdi N, Firat T, Ayhan Ç, Leblebicioglu G. Neurodynamic mobilization in the conservative treatment of cubital tunnel syndrome: long-term follow-up of 7 cases. J Manip Physiol Ther; 2010.33(3):156–63. DOI: 10.1016/j.jmpt.2009.12.001
Nagrale AV, Patil SP, Gandhi RA, Learman K. Effect of slump stretching versus lumbar mobilization with exercise in subjects with non-radicular low back pain: a randomized clinical trial. J Man Manip Ther; 2012.20(1):35–42. DOI: 10.1179/2042618611Y.0000000015
Ellis RF, Hing WA. Neural mobilization: a systematic review of randomized controlled trials with an analysis of therapeutic efficacy. J Man Manip Ther; 2008.16(1):8–22. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/19119380
Bertolini GRF, Artifon EL, Silva TS da, Cunha DM, Vigo PR. Low-Level laser therapy, at 830 nm, for pain reduction in experimental model of rats with sciatica. Arq Neuropsiquiatr; 2011.69(2–B):356–9. DOI: 10.1590/S0004-282X2011000300017
Bertolini GRF, Silva TS, Trindade DL, Carvalho AR. Neural mobilization and static stretching in an experimental sciatica model – an experimental study. Rev Bras Fisioter; 2009.13(6):2–7. DOI: 10.1590/S1413-35552009005000062
Ellis A, Bennett DLH. Neuroinflammation and the generation of neuropathic pain. Br J Anaesth; 2013.111(1):26–37. DOI: 10.1093/bja/aet128
