NEURAL PAIN PATHWAY TRACING OF RABBIT ISCHEMIC HEART BY DOUBLE-RETROGRADE NEUROTRACING

Theodorus Dapamede, Obed Trinurcahyo Kinantyo Paundralingga, Masruroh Rahayu, Bambang Soemantri
  MNJ, pp. 12-16  

Abstract


Background. Myocardial ischaemia leads to angina pectoris or referred pain, whichhappens because of the inability of the brain to distinguish the visceral afferent inputs from the somatic afferent inputs since they run along a common pathway via the dorsal root ganglia.
Objective. To distinguish specific areas of the rabbit heart that are projected to specific dorsal root ganglia, which then associates to its specific dermatomes.
Methods. A double-retrograde neurotracing method was used, with True Blue and Nuclear Yellow as the neurotracers. Rabbits were divided into 3 groups, which the first and second groups were ligated at the left anterior descending artery and at the left circumflex artery, respectively.The third group acted as the control group, without ligation.
Results. There is significant association between the site of ligation to the projection of the neurotracers at specific dorsal root ganglia (p<0.05). The first group showed high tendency to be projected to T2 and the second group showed a high tendency to project to T1.
Conclusion. This study shows that the rabbit heart can be specifically projected neuronally to specific dorsal root ganglia, following coronary artery ligation.

Keywords


myocardial ischaemia; neurotracing; referred pain

Full Text:

PDF

References


WHO. Contribution of Various Disease Categories to Global Mortality. Geneva: WHO, 2004.

AHA. Cardiovascular Disease in the United States. Dallas: American Heart Association, 2008.

WHO. The Impact of Chronic Disease in Indonesia. Geneva: WHO, 2002.

Sabatine MS and Cannon CP. Approach to Patient with Chest Pain. Braunwald's Heart Disease: A Textbook of Cardiovascular Medicine. 9th ed. Philadelphia: Elsevier Saunders, 2012, p. 1076-7.

White JC. Cardiac pain: anatomic pathways and physiologic mechanisms. Circulation. 1957; 16: 644-55.

Oztas E. Neuronal Tracing. Neuroanatomy. 2003; 2: 2-5.

Hu N, Straub CM, Garzarelli AA, Sabey KH, Yockman JW and Bull DA. Ligation of the left circumflex coronary artery with subsequent MRI and histopathology in rabbits. Journal of the American Association for Laboratory Animal Science : JAALAS. 2010; 49: 838-44.

Xiong G, Ling L, Nakamura R and Sugiura Y. Retrograde tracing and electrophysiological findings of collateral sprouting after end-to-side neurorrhaphy. Hand surgery: an international journal devoted to hand and upper limb surgery and related research: journal of the Asia-Pacific Federation of Societies for Surgery of the Hand. 2003; 8: 14550.

Richardson RJ, Grkovic I, Allen AM and Anderson CR. Separate neurochemical classes of sympathetic postganglionic neurons project to the left ventricle of the rat heart. Cell and tissue research. 2006; 324: 9-16.

Hopkins DA and Armour JA. Ganglionic distribution of afferent neurons innervating the canine heart and cardiopulmonary nerves. Journal of the autonomic nervous system. 1989; 26: 213-22.

Guic MM, Kosta V, Aljinovic J, Sapunar D and Grkovic I. Characterization of spinal afferent neurons projecting to different chambers of the rat heart. Neuroscience letters. 2010; 469:314-8.

Kuo DC, Oravitz JJ and DeGroat WC. Tracing of afferent and efferent pathways in the left inferior cardiac nerve of the cat using retrograde and transganglionic transport of horseradish peroxidase. Brain research. 1984;321: 111-8.

Sargowo D and Supriadi P. The Variation of Chest Pain and Masuk Angin Syndrome on Acute Myocardial Infarction in Saiful Anwar Hospital, Sub Urban Area Malang-Indonesia. Malang: Saiful Anwar Hospital, 1980.

Snell RS. Clinical Anatomy by Regions. 8th ed. Philadelphia: Lippincott Williams & Wilkins, 2008.


Refbacks

  • There are currently no refbacks.