UCAO (UNILATERAL CEREBRAL ARTERY OCCLUSSION) METHOD INCREASES THE LEVEL OF MMP- 9 BRAIN TISSUE IN RATS MODEL OF ISCHEMIC STROKE

Muhamad Rasjad Indra, Cakra Parindra Gasmara
  MNJ, pp. 47-51  

Abstract


Background. UCAO (Unilateral Cerebral Artery Occlusion) is a stroke induction method by ligating mice’s carotid artery for 45 minutes. Thus giving a hypoxic condition similar to stroke attacks to human. This method is less complicated and far more efficient. MMP-9 is a stroke marker which is assayed by ELISA from the blood of test animal.
Objective. To prove UCAO method is capable to raise MMP-9 concentration in mice’s blood.
Methods. This research was an experimental laboratory research with post-test only controlled group design. 8 male rats (8-10 weeks) were divided into 2 groups. A day after the treatment group had been induced to stroke, both group were tested to measure the MMP-9 blood concentration through ELISA.
Results. In this research, UCAO method had increased MMP-9 blood concentration in treatment group, compared to the control group. It is proved by the statistic tests, Mann-Whitney and Kruskal-Wallis, which showed a significant increase in treatment group (p < 0.05).
Conclusion. UCAO method is accepted as a method to create an ischemic stroke mice model.



Keywords


ischemic stroke; ischemic stroke mice model; ischemic stroke induction method; UCAO; MMP-9

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References


Cruz-Flores, S., et al. Racial-ethnic disparities in stroke care: the American experience: a statement for healthcare professionals from the American Heart Association. American Stroke Association. Stroke. 2011 42(7) : 2091116

Yayasan Stroke Indonesia. 2011. Jakarta, Republik Indonesia.

World Health Organization. 2011. The International Agenda for Stroke Marc Fisher, MD, University of Massachussets. Stroke AHA/ASA. Norrving B. 1st Global Conferences on Healthy Lifestyles and Noncommunicable Diseases Control. Moscow.

Zieve, D. 2011. Stroke.Neurosurgery, CedarsSinai Medical Center. National Library of Medicine. Los Angeles.

Caplan, A. 2007.Adult mesenchymal stem cells for tissue engineering versus regenerative medicine.J cell physiol. 213(2): 341-347.

Soyuer, F. 2005. Ischemic Stroke: Motor Impairment And Disability With Relation To Age And Lesion Location. The Internet Journal of Neurology. 3(2): DOI: 10.5580/9b4.

Mayo Clinic. 2012. Stroke: Treatment and Drug. http://mayoclinic.com/health/stroke/DS00150/DSECTION=treatments-and-drugs. Diakses pada tanggal 13 Oktober 2012.

Kuraoka, M., Furuta, T., Matsuwaki, T., Omatsu, T., Ishii, Y., Kyuwa, S., Yoshikawa, Y. Direct Experimental Occlusion of the Distal Middle Cerebral Artery Induces High Reproducibility of Brain Ischemia in Mice. Exp. Anim. 2009. 58(1):19-29.

Justicia, C., Panes, J., Sole, S., Cervera, A., Delofeu, R., Chamorro, A., Planas, A.M. Neurotrophil Infiltration Increase Matrix Metalloproteinase-9 in the Ischemic Brain After Occlusion/Reperfusion of the Middle Cerebral Artery in Rats. J Cereb Blood Flow Metab.2003. 23(12):1430-1440.

Ruhul Amin AR, Senga T, Oo ML, Thant AA, Hamaguchi M. Secretion of matrix metalloproteinase-9 by the proinflammatory cytokine, IL-1beta: a role for the dual signalling pathways, Akt and Erk. Genes Cells. 2003 Jun; 8(6):515-23.

Ma Z, Shah RC, Chang MJ, Benveniste EN. Coordination of cell signaling, chromatin remodeling, histone modifications, and regulator recruitment in human matrix metalloproteinase 9 gene transcription. Mol Cell Biol. 2004 Jun; 24(12):5496-509.

Sjahrir, H. 2003. Stroke Iskemik, Yandira Agung, Medan, p. 1-3.

Reynolds, M.A., Kirchick, H.J., Dahlen, J.R., Anderberg, J.M., Mcpherson, P.H., Nakamura, K.K., Laskowitz, D.T., Valkirs, G.E., Buechler, K.F. Early Biomarkers of Stroke.Clinical Chemistry.2003. 49(10):1733-1739.


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